叶 青

1999年-2003年 天津大学 精密仪器与光电子工程学院 本科/学士

2003年-2008年 南开大学 物理科学学院研究生/博士

2011年1月至今 南开大学 物理科学学院  副教授

2008年7月-2010年12月 南开大学 物理科学学院   讲师

    叶青，南开大学物理科学学院，副教授，博士生导师。2008年获得南开大学理学博士学位,同年留校任教，2015-2018年在美国Salk Institute进行访问研究，主要从事生物医学光子学相关领域及光电检测等方面的工作，主要涉及生物传感和生物成像等方向。

主持国家自然科学基金面上项目“折射率显微镜技术及其应用研究”，横向课题“压力变送与计量系统”、“四通道特定蛋白分析仪的开发”。国家质量基础的共性技术研究与应用专项“医用光学与工程光学关键计量标准及溯源方法研究”子课题负责人；天津市科技支撑计划“重要医学媒介生物病原体检测芯片体系的开发与应用”子课题负责人，科技部国际合作项目“新型乳腺癌早期预警系统的建立及防治方法的合作研究”子课题负责人，作为第二负责人参与研究天津市科技支撑计划“低成本床旁快速诊断系统的研究与开发”，参与研究国家重大科学仪器设备开发专项“单光子时间分辨成像光谱仪研发与应用”，“医用生物芯片检测分析系统的研究与开发”和“多功能OCT成像系统”等天津市自然科学基金项目。

本课题组常年招收光学、凝聚态方向博士生和硕士生，也欢迎本科材料、生物物理、信息、电子等方向的学生报考，常年开放一定的本科生创新项目课题，有意请联系: yeqing@nankai.edu.cn。

本课题组常年招收光学、凝聚态方向博士生和硕士生，也欢迎本科材料、生物物理、信息、电子等方向的学生报考，常年开放一定的本科生创新项目课题，有意请联系: yeqing@nankai.edu.cn。

1. Biomedical Photonics imaging

Biomedical photonics provides solutions for medicine, biology and biotechnology based on photonics. Biomedical photonics contains the imaging and detection for biological materials and achieving detection, diagnosis and treatment for disease on both a macro and a micro level. Our major work is constructing the system of biophotonic sensing and imaging, such as scanning focused refractive-index microscopy, which can be applied to medical diagnosis and biological sensing.

Scanning focused refractive-index microscopy

2. Photoelectric Detection

Photoelectric detection is applying novel optical detection methods and technologies into particular detection combining modern mechanical and electric technology. Our main research is developing lateral flow test strip reader based on embedded system and system of measuring parameters of end faces of steel pipes.

Lateral flow test strip reader based on embedded system

Lateral flow immunoassay is a widely used kind of rapid detection method. With conventional reporters, it could be used in quantitative detection or semi-quantitative detection of many analytes including antigens, antibodies, and even the products of nucleic acid amplification tests.
Nowadays, most of the test results are read out by human eyes. To determine the numerical value of the fluid concentration, the operator should compare it to the standard colorimetric card. As the inevitable difference between human eyes and the light circumstances, it’s difficult to avoid errors. Two operators may have two different opinions towards one test result, especially when the test result is near the cut-off value. This could be reduced by employing test strip reader. The research on developing the test strip reader has already begun. The typical reader is built by light source and optical detector that measures the light reflected from the strip based on the absorbance detecting method.

We have designed and fabricated a new lateral flow test strip reader based on ARM processor and embedded Linux operating system. The instrument uses green light-emitting diode as the light source, captures the test strip image with high-resolution CMOS image sensor, and then use the image-processing method to calculate the result. It has many advantages such as rapid, easy-to-use and reliable. When testing cocaine fluid of different concentrations, the analysis result of test strips shows that the accuracy is 1ng/ml and CV (coefficient of variation) is less than 3%. The instrument could also be used on other kinds of lateral flow strips.

3. System for measuring of the parameters of the end faces of the steel pipes

In order to realize automatic non-contact measurement for pipe end inside diameter, outside diameter and ellipticity, while meeting the detection needs of seamless steel pipe production and processing enterprises, a pipe end inside and outside diameter measurement system is designed and implemented. First, use two laser optical triangulation sensors to perform a full scan of the inside and outside diameter as they rotate around the pipe ends. Then use fitting algorithm to reduce errors brought by the no coincidence of rotary axis and steel pipe axis. Motorized translation stages which are fixed on the sensor mounting arm, and a lift are provided at the same time to meet the measurement needs of different specifications of steel pipes. Experimental results indicate that the system measurement accuracy is less than 0.05mm; repeat accuracy is 5um; one measurement process lasts less than 25 seconds while 500 points are sampled per pipe end. It can fully meet the requirements of seamless steel pipe production enterprises, with advantages of high precision, easy operation and rapid speed. The system has passed the off-line test in the Tian Guan Yuan Tong pipe products Co. Ltd, Tianjin.

4. Study of the mechanism of optical clearing

‍‍The application of optical clearing technique can reduce the scattering coefficient and enhance the penetration depth of light in biotissues. It can achieve better optical efficiency in biomedical application. We establish a theoretical model of the measurement of optical parameters of bio-tissues under the total internal reflection method. After the application of the optical clearing technique, on the interface of bio-tissues, the refractive index matching status between cells' internal and external environments can be studied.

2010年-2013年，基础物理实验（电磁学），128课时/年

2011年-2015年，电子技术基础（二），54课时/年

2013年-2015年，机械制图，36课时/年

指导本科毕业论文14人

指导本科生“百项工程”，国家大学生创新性实验计划项目等项目；

[1]2016年，指导南开大学第十四届百项工程， “基于光层析成像技术对光通透的研究”，指导教师：叶青，学生：王晶，冯春晗，李文爽，张云帆 。

[2]2016年，指导南开大学第十四届百项工程， “基于傅里叶相干衍射层叠成像显微技术的生物组织成像研究”，指导教师：叶青，学生： 乐应波，段昆龙，徐丽琳。

[3]2015年，南开大学“国家级大学生创新创业训练计划”项目， “基于柱坐标系的3D打印机的研究与开发”，指导教师：叶青，学生： 张家强，张泽昊，周砚柠

[4]2014年，南开大学“国家级大学生创新创业训练计划”项目，便携式多通道PCR仪的研究与开发”，指导教师：叶青，梅剑春，学生：杨麒麟，崔曦雯，张尚

[5]2013，指导南开大学第十届百项工程，“基于全内反射法的粉末折射率测量研究”，指导教师：叶青，学生：杨凌雨，刘婧

[6]2013，指导南开大学第十届百项工程，“基于全内反射法的折射率空间分布分布测量研究”，指导教师：叶青，学生：余建芳

[7]2012年，指导南开大学第九届百项工程， “基于RFID技术的超市管理系统”，指导教师：叶青，学生：雷星，李靖，马丁，周涵，黄文铮，优秀奖。

[8]2012年，指导南开大学第九届百项工程，“四通道特定蛋白分析仪的设计与研发”，指导教师：叶青，学生：贾晓菲，杜芳美，孙腾骞，姜晶殷，潘玉松。获校级二等奖。

[1] 2014年，南开大学2014年教学改革项目，“物理学类《机械制图》课程教学方法改革与实践”， 主持。

[2] 2014年，南开大学2014年教学改革项目，“大学物理基础实验——声波信号提取与应用”，参与。

[3] 2013年，南开大学青年教师教改项目，“《电子技术基础》课程教学方法改革与实践”，主持。

主持及负责：

1，国家质量基础的共性技术研究与应用专项“医用光学与工程光学关键计量标准及溯源方法研究”子课题负责人，2015.12-2020.12，

2，天津市应用基础与前沿技术研究计划项目，折射率显微镜技术及其应用研究，主持，2015.4-2018.3

3，国家自然科学基金面上项目, 折射率显微镜技术及其应用研究，主持，2015.1-2018.12

4，科技部国际合作项目，新型乳腺癌早期预警系统的建立及防治方法的合作研究，子课题负责人，2012.6-2015.11

5，钢管制造信息化系统及管端参数测量系统的研究与开发，子课题负责人，2012.4-2014.10

6，四通道特定蛋白分析仪的开发，主持，2013.1-2014.12

7，NS5001免疫层析结果判读记录仪，主持，2015.6-2015.9

8， 手持式食品安全检测仪的研制，主持，2015.5-2015.8

主要参与：

1，重大科学研究计划，关联光子学微结构的非线性光学特性与调控机理研究，2011.1-2015.12

2，国家重大科学仪器设备开发专项，单光子时间分辨成像光谱仪研发与应用，2013.10-2018.9

3，天津市自然科学基金，低成本床旁快速诊断系统的研究与开发，2011.4-2013.9

4，天津市自然科学基金，多功能OCT成像系统，2005.1-2008.6

5，天津市自然科学基金，医用生物芯片检测分析系统的研究与开发 2003.1-2005.12

Publications

40. Zhichao Deng, Jin Wang,Hu, Zhixiong Hu, Jianchun Mei, Shike Liu,Ningning Huang, Qing Ye* & Jian-Guo Tian. “Complex Refractive Index Dispersion of Strong Absorbing Material Determined Using Internal Reflectance Spectra Measurement”， Applied Spectroscopy，72(9）,（2018）

39. Zhichao Deng, Jin Wang, Qing Ye, Tengqian Sun, Shike Liu, Wenyuan Zhou, Chunping Zhang, Jianchun Mei, Jianguo Tian, “A method for measuring the continuous dispersion spectrum of nonlinear refraction coefficients of materials,” Optics and Laser Technology,98(2018), 252–256 (2017)

38 Zhi-Chao Deng, Jin Wang, Zhi-Xiong Hu, Jian-Chun Mei & Qing Ye，“Study on the relation between the refractive index of fresh muscle tissue and its water content from 400 to 750 nm,” Journal of Modern Optics ,1362-3044，(2017)

37 Xiaowei Jin, Zhichao Deng, Jin Wang, Qing Ye*, Jianchun Mei, Wenyuan Zhou, Chunping Zhang, Jianguo Tian, Study of the inhibition effect of thiazone on muscle optical clearing, J. Biomed. Opt. 21(10), 105004 (2016).

36 Teng-Qian Sun ; Qing Ye* ; Fen Hu ; Shi-ke Liu ; Xiao-Wan Wang ; Jin Wang ; Zhi-Chao Deng ; Jian-Chun Mei ; Wen-Yuan Zhou ; Chun-Ping Zhang ; Xin-Yu Wang ; Lei-Ting Pan ; Jian-Guo Tian，Quantitative index imaging of coculture cells by scanning focused refractive index microscopy，J. Biomed. Opt. 21(8), 086016, 2016.

35 Tengqian Sun, Qing Ye*, Shike Liu, Xiaowan Wang, Jin Wang, Zhichao Deng, Jianchun Mei, Wenyuan Zhou, Chunping Zhang, and Jianguo Tian. Measurement of complex refractive index of turbid media by scanning focused refractive index, Optics Letters, 41(16):3767-3770, 2016.

34 Tengqian Sun, Qing Ye*, Xiaowan Wang, Jin Wang, Zhichao Deng, Shike Liu, Qilin Yang, Jianchun Mei, Wenyuan Zhou, Chunping Zhang, and Jianguo Tian. Time dependent detection of the refractive index distribution of nonlinear optical materials. IEEE Photonics Technology Letters, 28(3), 260-263, 2016.

33 Zhichao Deng, Jin Wang, Qing Ye*, Tengqian Sun, Wenyuan Zhou,  Jianchun Mei, Chunping Zhang, and Jianguo Tian. Determination of continuous complex refractive index dispersion of biotissue based on internal reflection. Journal of Biomedical Optics, 21(1), 015003, 2016.

32 Jin Wang, Zhichao Deng, Xiaowan Wang, Qing Ye*, Wenyuan Zhou, Jianchun Mei, Chunping Zhang, and Jianguo Tian. Measurement of the refractive index of hemoglobin solutions for a continuous spectral region. Biomed. Opt. Express, 6(7), 2536-2541, 2015.

31 Yu Zheng, Qing Ye*, Chen Wang, Jin Wang, Zhichao Deng, Jianchun Mei,Wenyuan Zhou, Chunping Zhang, and Jianguo Tian. Nonlinear optical properties of methyl red under CW irradiation. Optics & Laser Technology, 75, 132–137, 2015.

30. Zhichao Deng, Jin Wang, Yuanze Liu, Qing Ye*, Tengqian Sun, Wenyuan Zhou, Jianchun Mei, Chunping Zhang, Jianguo Tian. Research on the change of complex refractive index of porcine muscle during natural dehydration. Journal of Biomedical Optics, 2015, 20(4), 045001.

 29.  Jiwen Sun, Jin Wang, Yuanze Liu, Qing Ye*, Huanhuan Zeng, Wenyuan Zhou, Jianchun Mei, Chunping Zhang, Jianguo Tian. Effect of the gradient of complex refractive index at boundary of turbid media on total internal reflection. Optics Express, 2015, 23(6), 7320-7332.

 28.  Zhichao Deng, Jin Wang, Qing Ye*, Tengqian Sun, Wenyuan Zhou, Jianchun Mei, Chunping Zhang, Jianguo Tian. Continuous refractive index dispersion measurement based on derivative total reflection method. Review of Scientific Instruments, 2015, 86, 043101.

 27.  Xiaowan Wang, Qing Ye*, Tengqian Sun, Jin Wang, Zhichao Deng, Jianchun Mei, Wenyuan Zhou, Chunping Zhang, Jianguo Tian. Two-dimensional scanning focused refractive-index microscopy and applications to refractive-index profiling of optical fibers. Review of Scientific Instruments, 2015, 86, 013702.

 26.  Huanhuan Zeng, Jin Wang, Qing Ye*, Zhichao Deng, Jianchun Mei, Wenyuan Zhou, Chunping Zhang, Jianguo Tian. Study on the refractive index matching effect of ultrasound on optical clearing of bio-tissues based on the derivative total reflection method. Biomedical Optics Express, 2014, 5(10), 3482-3493.

 25.  Tengqian Sun, Qing Ye*, Xiaowan Wang, Jin Wang, Zhichao Deng, Jianchun Mei, Wenyuan Zhou, Chunping Zhang, Jianguo Tian. Scanning focused refractive-index microscopy. Scientific Reports, 2014, 4:5647. 

 24.  Jin Wang, Qing Ye*, Zhichao Deng, Wenyuan Zhou, Chunping Zhang, Jianguo Tian. Note : Refractive index as an indicator for non-homogeneous solid identification. Review of Scientific Instruments, 2014, 016102. 

 23.  Jin Wang, Qing Ye*, Zhichao Deng, Wenyuan Zhou, Jianchun Mei, Chunping Zhang, Jianguo Tian. Application of matching liquid on the refractive index measurement of biotissue: A theoretical and experimental study. Optics Communications, 2014, 319: 36-41.

 22.  Jianchun Mei, Qing Ye*, Jianguo Tian. Design of measurement system for inside and outside diameters of steel pipe. Optics and Precision Engneering(in Chinese), 2014, 22(4):815-821.

 21.  Jin Wang, Qing Ye*, Zhichao Deng, Tengqian Sun, Wenyuan Zhou, Jianchun Mei, Chunping Zhang, Jianguo Tian. Study of dynamic pressure-induced refractive index change using derivative total reflection method. Journal of Biomedical Optics, 2013,18(11),117005 

 20.  Qing Ye, Jin Wang, Zhibo Liu, Zhichao Deng, Xiangtian Kong, Fei Xing, Xudong Chen, Wenyuan Zhou, Chunping Zhang, Jianguo Tian, Polarization-dependent optical absorption of graphene under total internal reflection, Applied Physics Letters, 2013, 102, 021912 

 19.  Qing Ye, Wenyuan Zhou, Jin Wang, Chunping Zhang, Jianguo Tian. Study on effective probe depth of optical coherence tomography system by Monte Carlo simulation. Optik, 2013, 124, 4909-4911.

 18.  Cuifeng Ying, Wenyuan Zhou, Yi Li, Qing Ye, Na Yang, Jianguo Tian. Multiple and colorful cone-shaped lasing induced by band-coupling in a 1D dual-periodic photonic crystal. AIP Advances, 2013, 3(2), 022125.

 17.  Cuifeng Ying, Wenyuan Zhou, Qing Ye, Yi Li, Na Yang, Jianguo Tian. Miniband lasing in a 1D dual-periodic photonic crystal. Laser Physics Letters, 2013, 10(5), 056001. 

 16.  Zhili Li, Wenyuan Zhou, Yange Liu, Qing Ye, Yue Ma, Huifeng Wei, Jianguo Tian. Highly efficient fluorescence detection using a simplified hollow core microstructured optical fiber. Applied Physics Letters, 2013, 102, 011136. 

 15.  Jin Wang, Qing Ye*, Zhichao Deng, Wenyuan Zhou,Tengqian Sun, Chunping Zhang, Jianguo Tian. Effect of tissue fluid on determination of the complex refractive index of animal tissue. Journal of Biomedical Optics, 2012, 17(7), 075011.

 14.  Jin Wang, Qing Ye*, Zhichao Deng, TengqianSun, Wenyuan Zhou, Chunping Zhang, Jianguo Tian. Estimation of the complex refractive index of epoxy resin phantom using EDTRM. Journal of Modern Optics, 2012, 59(13), 1158-1162.

 13.  Jin Wang, Qing Ye*, Zhichao Deng, Wenyuan Zhou, Chunping Zhang, Jianguo Tian. Accurate determination of the complex refractive index of solid tissue-equivalent phantom, SPIE Proceedings, 2012, 842729.

 12.  Cuifeng Ying, Wenyuan Zhou, Qing Ye, Zubin Li, Jianguo Tian. Mini-band states in graded periodic 1D photonic superstructure. Applied Physics B: Lasers and Optics, 2012, 107 (2):369-374. 

 11.  Fei Xing, Zhibo Liu, Zhichao Deng, Xiangtian Kong, Xiaoqing Yan, Xudong Chen,  Qing Ye, Chunping Zhang, Yongsheng Chen, Jianguo Tian, Sensitive Real-Time Monitoring of Refractive Indexes Using a Novel Graphene-Based Optical Sensor, Scientific Reports, 2012, 2, 908.

 10.  Qing Ye, Jin Wang, Zhichao Deng, WenyuanZhou,Chunping Zhang, Jianguo Tian. Measurement of the complex refractive index of tissue-mimicking phantoms and biotissue by extended differential total reflection method. Journal of Biomedical Optics, 2011, 16(9), 097001.

 9.  Jianchun Mei, Qing Ye*, Wenyuan Zhou, Wangwei Hui, Jianguo Tian. Development and study of lateral flow test strip reader based on embedded system. Electronic Measurement & Instruments (ICEMI), 2011 10th International Conference on. IEEE, 2011, 1: 201-204.

 8.  Qing Ye, Fuxin Li, Yu Liu, Wenyuan Zhou, Chunping Zhang, Jianguo Tian. Dual-wavelength optical coherence tomography system. Laser & Optoelectronics Progress(in Chinese), 2010, 47, 041701. 

 7.  Cuifeng Ying, Wenyuan Zhou, Qing Ye, Xiaoliang Zhang and Jianguo Tian. Band-edge lasing in Rh6G-doped dichromated gelatin at different excitations. Journal of Optics, 2010, 12:115101. 

 6.  Qing Ye, Fuxin Li, Yu Liu, Wenyuan Zhou, Chunping Zhang, Jianguo Tian. Refractive index measurement of biotissue based on Fourier domain optical coherence tomography. Journal of Optoelectronics&Laser(in Chinese), 2009, 20(1):126-129.

 5.  Qing Ye, Yu Liu, Wenyuan Zhou, Jianguo Tian. Design and implementation of lens for fast biochip detection system. SPIE Proceedings, 2007, 672247.

 4.  Qing Ye, Wenyuan Zhou, Yu Song, Jianguo Tian. Design and implementation of a low-cost system for real-time video acquisition and processing. Journal of Optoelectronics&Laser(in Chinese), 2006, 17(6):742-745.

 3.  Yu Song, Wenyuan Zhou, Qing Ye, Weiping Zang, Jianguo Tian. A high sensitivity optical multichannel analyzer. Spectroscopy and Spectral Analysis(in Chinese), 2006, 26(4), 775-778.

 2.  Guiying Li, Linqi Shi, Qing Ye, Wenyuan Zhou, Jianguo Tian. Electric-field-assisted assembly and alignment of polystyrene-b-poly(acrylic acid) micelles, Colloid and Polymer Science, 2006, 284(10), 1179-1183. 

 1.  Yu Song, Wenyuan Zhou, Lei Chen, Qing Ye, Jianguo Tian. Implementation of CCD high speed data acquisition by USB based DMA. Journal of Optoelectronics&Laser(in Chinese), 2004, 15(10):1181-1185.

专利：

1. 梅剑春，叶青，田建国，一种多线胶体金试纸条定量分析图像处理方法，2016.4.13授权，201310201407.6

2. 叶青，梅剑春，田建国，钢管管端内外径双臂四探头测量装置及测量方法，中国，发明专利，201210042474.3

3. 叶青，孙腾骞，王槿，邓志超，张春平，田建国，聚焦全内反射法测量物质折射率分布，2012.7，中国，201210062142.1

4. 马玉祥, 叶青, 胡新华, 田建国. 智能大功率半导体激光器驱动装置. 申请号：200610013955.6 . 已授权

5. 周文远, 叶青, 刘宇, 田建国. 用于快速生物芯片检测的成像镜头. 200610013956.0.

6. 叶青，梅剑春，田建国，多波长毛细管电泳荧光激发装置，2013.11.27授权，中国，201310201409.5

7. 叶青，孙腾骞，周文远，田建国，脉冲光信号接收放大器，2011.8.24授权，中国，201120001813.4

8. 梅剑春，周文远，叶青，田建国，电子延时安全插座，2012.4.11授权，中国，201120143055.x

9. 梅剑春，叶青，田建国，光纤式小体积金标免疫层析试纸检测仪，2013.10.9授权，中国，201320295195.8

10. 周文远, 叶青, 田建国.实时视频采集处理系统. 2006.7.26授权，中国200520026603.5.

11. 马玉祥, 叶青, 胡新华, 田建国.数字式大功率半导体激光器驱动装置. 200620026204.3.

12. 叶青，周文远，刘宇，田建国，多波长 OCT 系统的聚焦镜头，2009.5.20授权，中国，200820075057.8

13. 叶青，周文远，田建国，双波长OCT系统，2009.5.20授权，中国，200820075056.3

14. 叶青，邓志超，王槿，孙腾骞，张春平，田建国，基于全内反射法的宽光谱范围物质色散的自动测量装置，中国，201310524601.8

15. 叶青，邓志超，王槿，孙腾骞，张春平，田建国，磁颗粒荧光聚集检测装置，中国，201310524507.2

16. 叶青，梅剑春，田建国，双光源照明金标免疫层析试纸检测分析方法，2013.5，中国，201310201410.8

17. 叶青，邓志超，王槿，孙腾骞，张春平，田建国，基于全内反射的混合物折射率测量方法，2012.3，中国，201210062141.7

18. 叶青，周文远，田建国，基于频域 OCT 的生物组织折射率测量方法，2010.6，中国，200810053555.7

19. 叶青，周文远，田建国，多波长 OCT 系统，2010.6，中国，200810053556.1

20. 张春平, 田建国, 郭磊, 叶青, 傅汝廉. 牛角形光导管装置. 申请号：200720096496.2.

2014年南开大学青年教师教学基本功竞赛，最佳选手

2014年天津市第十二届高校青年教师教学基本功竞赛理科组三等奖。

2014年8月，27届世界青年物理学家竞赛（IYPT，The 27^th international Young Physicists' Tournament，Shrewsbury，UK，学生名单：梁艳，赵亦俢，刘睿豪，汪涵伟，陆思聪）领队，金奖。