Imshik Lee

Ph.D in Bio(Polymer)-Physics H.H.Wills Physics Laboratory, University of Bristol, UK. Sept. 1989 – Oct. 1992 B.Sc. in Physics Pusan National University, Korea Mar. 1982 – Feb. 1986

April. 2003 – Present Professor(Outstanding Professor; 2005~) TEDA Applied Physics School & Department of Biophysics, School of Physics, Nankai University, Tianjin, China Aug. 2002 – March 2003 Visiting Professor Tokyo Medical and Dental University, Japan July 1998 – July 2002 Sr. Researcher Department of Biomedical Engineering, Case Western Reserve University, USA July 1995– June 1998 Research Fellow - Department of Chemistry, University of Hong Kong, Hong Kong (June 1997-July 1998) - Institute of Chemistry, Chinese Academy of Sciences, Beijing, P.R.China (July 1995-June 1997) Dec. 1992 – June 1995 Assistant/Associate Professor Yanbain University of Science & Technology, Jilin, P.R.China Oct. 1991 – April 1992 Research assistant H.H. Wills Physics Laboratory, University of Bristol, UK

Nanobiointerfaces

I have beeninterested in three main fields, bio-interfaces,physio-(eg. Mechanical / electromagnetic)interfaces, and chemo-interfaces;

1.Bio-interfaces:Nanotechniques make a possible to study singlemolecular kinetics at thenanobiointerface including ligand-receptor interactions. Applying dynamicmeasurement of the interaction, energy landscape could be understood at theligand-receptor binding. (Example: Single molecular GPIIb3a-RGD ligandinteraction, Surf. Sci 2001, 491: 433-443)

2.Chemo-interfaces: Biomimetic and bio-pattern studies: designing Biomimetic molecules and characterizing theirinterfacial properties at the single molecular level. The cellularnano-interfaces were also applied in controlling cell growth patterns.(Example:Biomimetic nanohydrogel, Biomaterials, 2004, 25,1911-1918). This work included the computer modeling and molecular designs.

3.Physio-interfaces:The conventional stimuli were considered only in biochemical stimulus. Myresearch investigates the physical stimuli such as mechanical, optical(electromagnetic) and thermal stimuli at the cells. How the cells respondingagainst physical stimuli is studying with combination of optical andbiophysical techniques. Another crucial question is how the physical stimuliwere converted into the physiological signals. From conventional staticphysical stimuli to dynamic stimuli is on characterizing. Those results willbuild a new paradigm in controlling biological functions with physicalstimulus. My current research topics were;

-Mechanotransduction; Mechanical stimuli interact directly with cytoskeleton dynamicsand submembrane molecules, which are correlated with the physiologicalfunctions. Due to the dynamic responses of a living cell, dynamic stimuli interms of frequency, resting intervals, and amplitude are applied andcharacterized. Micro-fluid multishear channel was applied on this approach.(Example: Fig  Microfluid mulitshearstress channels, BBRC 2011, 408, 350–355)

-(Ir)radiation transduction; Optical sources were very good tools to monitor thebiological activities. However, it has not been considered, as an opticalsource itself is a stimulus in triggering the biological function. (Example:Fig. Frequency and intensity dependent signal, a) Empirical devices, BBRC 2010, 396, 662-666and b) Ca²⁺ signal of our theoretical model, Chin Phys Lett, 2009.26,1 017102).

-Bio-signal pattern analysis: Bio-signals exposing the specified biological functionswere interested to de-cord. Biological and biochemical functions werecorrelated with the physical biosignals. The biosignals were attempted todetect by applying nano-senses, and obtained the signals were decording tocharacterize the corresponding biological functions.  

Molecular Biophysics (Undergraduate)

Nanobiophysics(Graduate)

Theoretical Biophysics (Graduate)

Biophysics (Undergraduate)

NSFC(National Science Foundation ofChina,2012-15)

NSFC(2011-13) PI

TJBSF(Tianjin Basic ScienceFoundation, 2007-10) PI

NSFC( 2006-7) PI

Ministry of Education, China (PhD Supervisor Fund, 2005-7) PI

Fund for the outstanding Prof.,Nankai Univ. ( 2003.4-2005.3); PI

Japanese Ministry of Sport,Culture and Education 2002.7-2003.9); PI

70. P Jiang; F Xing; B Guo; J Yang; ZLi; F Hu; I Lee; X Zhang; L pan; J Xu(2017) Nucleotide transmitters ATP andADP mediate intercellular calcium wave communication via P2Y12/13 receptorsamong BV-2 microglia. PLoS One 2017 11;12(8):e0183114. Epub

69. Y Cao, X Wu, R Yang, X Wang, H Sun, I Lee (2017)Self-assembling Study of Sarcolipin and Its Mutants in Multiple MolecularDynamic Simulations. Proteins, doi:10.1002/prot.25273

68.Y Cao, XWu, X Wang, H Sun, I Lee (2016) Transmembrane dynamics of the Thr-5 phosphorylatedsarcolipin pentameric channel. Archives ofBiochemistry and Biophysics. 604:143–151.

67. G Nan, Y Zhang, S Li, I.Lee, T takano, S Liu.(2016) NaCl stress-induced transcriptomicsanalysis of Salix linearistipularis (syn. Salix mongolica). Biol Res Thesal. DOI10.1186/s40709-016-0038-7

66. Y Cao, X Wu, I Lee*, X Wang (2016)Molecular dynamics of water and monovalent-ionstransportation mechanisms of pentameric sarcolipin.Proteins.84:73-81. doi: 10.1002/prot.24956.

65. F Hu, F Xing,G Zhu, G Xu, C Li, JQu, I Lee, L Pan (2015) Rhein antagonizes P2X7 receptor in rat peritoneal macrophages. Scientific Reports09/2015;5:14012. DOI:10.1038/srep14012

64. C Yang, S Ma, I Lee, J Kim, S Liu(2015) Saline-induced Changes of Epicuticular Waxy Layer on the Puccinellia tenuifloraand Oryza sativa Leave Surfaces, Biological Research,48:33 (doi:10.1186/s40659-015-0023-x)

63. D Lee, J Lee, I.Lee, (2015) Cell Phone Generated RadioFrequency Electromagnetic Field Effects on the Locomotor Behaviors of theFishes Poecilia reticulata and Danio rerio, Int. J. Radiation Biology,91(10): 843-850.( DOI:10.3109/09553002.2015.1062575)

62. M Choe, W. Choe, I.Lee*, M Wu, S Liu(2015) ComputationalAnalysis of Mutated AHAS Appeared by Sulfonylurea (SU) Herbicides, WeedResearch, 55: 359-369.

61. Fen Hu, Leiting Pan, Kai Zhang, Fulin Xing, XinyuWang, Imshik Lee, Xinzheng Zhang, Jingjun Xu (2014) Elevation ofExtracellular Ca²⁺Induces Store-Operated Calcium Entry viaCalcium-Sensing Receptors: A Pathway Contributes to the Proliferation ofOsteoblasts. PLoS ONE, 9: e107217

60. YuanyuanBu, Bo Sun, Aimin Zhou, Xinxin Zhang, Imshik Lee andShenkui Liu (2013) Identification and characterization of a PutAMT1;1gene from Puccinellia tenuiflora(2013) PLoS ONE8(12): e83111

59. GuixianMeng; Leiting Pan, Cunbo Li; Fen Hu; Xuechen Shi; Imshik Lee; Irena Drevensek-Olenik;Xinzheng Zhang; Jingjun Xu, (2014) Temperature-induced Labelling of Fluo-3 AMSelectively Yields Brighter Nucleus in Adherent Cells, BBRC, 443;888-893

58.L Pan, K Song, W Sun, F Hu,I Lee*(2013) Nitric oxideinduces Ca²⁺ entry by activating TRPV1-like channel viaS-nitrosylation in osteoblasts: an implication of the pathogenesis ofinflammation-mediated osteoporosis.Eu.J Pharm, 715; 280-285

57. X Wu, L Pan, Y Liu, P Jiang,I Lee, I Drevensek-Olenik, X Zhang, J Xu(2013) Cell-cell communication induces random spikes of spontaneous calciumoscillation in multi-BV-2 microglial cells. BBRC, 431;664-669.

56. X Kong, J Zhang, X Han, P Zhang, X Dai,J Liu, X Zhang, I Lee, S Liu,(2013) High-Yield production in E. coli of fungal_immunomodulatory protein isolated from Flammulina velutipes and its bioactivity assay in vivo, Int. J. Mol. Sci., 14; 2230-41.

55. Gao C-Y, Meng GX, Li X, Wu M, Liu Y, Li XY, Zhao X, Lee I, Feng X (2013)Wettability of dragonfly wings: the structure detection and theoreticalmodeling, Surf. InterfaceAnal.,45; 650–655,

54. Pan.L, Wang X, Yang S, Wu X, Lee I, Zhang X, RuppRA, Xu J (2012) Ultraviolet Irradiation-Dependent Fluorescence Enhancement ofHemoglobin Catalyzedby ROS, PLoS ONE 7(8) e44142.

53. Pan L, Wu X, Zhao D, Hessari NM, Lee I, et al. (2011) SulfhydrylModification Induces Calcium Entry through IP3-Sensitive Store-Operated Pathwayin Activation-Dependent Human Neutrophils. PLoS ONE 6(10): e25262.

52. Y.Cui,Y.J. Oh,J.Lim, M.Youn,I..Lee,H.K.Pak,W.Park,W.Jo,S.Park, (2012) AFM studyof the differential inhibitory effects of the green teapolyphenol () epigallocatechin3gallate (EGCG) against Gram-positive and Gram-negative bacteria, FoodMicrobiology, 29, 80-87

51. S Kou, L Pan,D van Noort, G Meng, X Wu, H Sun, J Xu,I Lee*, (2011) A microfluidicmultishear device for quantitative analysis of Calcium in osteoblasts,BBRC, 408 (2011) 350–355

50. S Kou, G Meng,L Pan, X Wang, Z Ju,I Lee* (2012)Computation Simulation and Application for Novel Microfluidic MultishearDevice, Acta ScientiarumNaturalium Universitatis Nankaiensis,2012,45(2): 31~36

49．Ge Q, LeeI* (2011) Kinetics of Swelling/ShrinkingRearrangement of a Self-aggregated Nanohydrogel at Solid/Liquid , J Exp. Nanosci., 6(5),509-520

48. X Zhang,X Liu, L Pan, I Lee*,（2010）Magnetic Fields at ExtremelyLow-Frequency (50 Hz, 0.8 mT) Can Induce the Uptake of Intracellular CalciumLevels in Osteoblasts, Biochem. Biophys. Res. Com. (BBRC),396, 662-666

47. LuoM, Song K, Zhang X, Lee I,(2010) Replyto Comment on Mechanism for Alternating Electric Fields Induced-Effects onCytosolic Calcium, Chinese Physics Letters, 27,049902.

46. X Liu, XZhang, and I Lee* (2010) A quantitative study onmorphological responses of osteoblastic cells to fluid shear stress. ActaBiochimica et Biophysica Sinica 42(3):195-201;

45. Lou M, Song k, Zhang X, Lee I* (2009)Mechanism for Alternating Electric FieldsInduced-Effects on Cytosolic Calcium, ChinPhys Lett, 26(1) 017102.

44. 罗明艳，陈梅宗，李任植*（2008）αl_lbβ₃同源模拟和环形RGD药物小分子设计. ChinJ Biotech, 24(2): 297-301

43.罗明艳，张旭，宋昆，李任植*(2009) 交变电场诱导胞浆内钙离子变化的机制。中国科技论文01-243

42. 罗明艳，宋昆，陈梅宗，李任植*(2009) 整合蛋白α_IIbβ₃的结构的同源模拟。中国科技论文，01-750

41.罗明艳，孙海英，宋昆，陈梅宗，李任植*(2009)环形x-RGD-y药物小分子设计、对接筛选。中国科技论文，01-982

40. XZhang, X Lu, J Sun, S He, I Lee*, H Pak, (2008) Real-time Observations of Nanopoint StimulusInduced Enhancements of Mechanical Properties in Osteoblast Cells, Ultramicroscopy 2008;108(10):1338-41.

39. M Luo, M Chen,I Lee*  (2008) α_IIbβ₃ modelingsimulation and design of the cyclic RGD, Chin.J of Biotechnology. 24(2), 297-301

38. SuJ, Liu Y, SongK, He S, Lee I, (2007)The study of the surface-enhanced Raman spectroscopy of osteoblast after thestimulation of electric currentCRTER,11(44), 8839

37 I Lee, H Sun, J Song, Y Zhang, (2008) Kinetics ofthe Self-assembled Nanohydrogels at a Hydrophobic Interface,JNN(Journal of Nanoscience and Nanotechnology), 83386-3391

36. K. Song, JY Su, SJ He,I.Lee*, (2007)Real-time Raman spectra of osteoblasts in pulsed electromagnetic field with lowfrequency and amplitude. CRTER (Clinical Rehabilitative TissueEngineering Research), 11(40), 8090-94

35. Zou ZQ, Liu YN, Wu Y, Li QN,Chen MZ, Sun HY,Lee I*.(2007) Surface-enhancedRaman spectra of oxidation damnification of fetal bovine serum by ozone, Spectroscopy and Spectral Analysis, 27(6) 1140-2 (in Chinese)

34. I.Lee*, K Kobayashi, HY Sun, S Takatani, LG Zhong, (2007) Biomembrane mimetic polymer Poly(2-methacryloyloxyethylphosphorylchroline -co-n-butyl methacrylate) at the Interface of Polyurethane Surfaces , JBiomat. Res.A 82, 316-22

33. I.Lee*, K-Y.Chan, and D.I.Phillips (2004) “2D Dendrites and 3D Growth ofElectrodeposited Platinum Nanocrystals” Japanese Journal of Applied Physics,43,767-770

32. I.Lee* and K. Akiyoshi (2004) “Single Molecular Mechanics of aCholesterol-Bearing Pullulan Nanogel at the Hydrophobic Interfaces”Biomaterials, 25, 1911-1918

31. I.Lee and R.E. Marchant, (2003) “Molecular interaction studies ofhemostasis: fibrinogen ligand-human platelet receptor interactions" Ultramicroscopy97, 341-352

30.R.E.Marchant, I.Kang, P.S.Sit, B.Tadd, S.J.Eppell and I.Lee(2002) “Molecular views and measurements ofhemostatic processes using atomic force microscope (Review article)” CurrentPeptide & Protein Science, 3(3), 249-274.

29. I.Lee and R.E.Marchant (2001)“Forcemeasurements on the molecular interactions between ligand (RGD) and humanplatelet α_IIbβ₃ receptorsystem” Surface Science. 491: 433-443

28. I.Leeand R.E.Marchant (2000)“DynamicDebonding Force Measurements of Ligand (RGD) and Platelet Receptor (GPllb/llla) System under Physiological Conditions.” Microscopy and Microanalysis,6, 974-975.

27.I.Lee, and R.E.Marchant (2000)"ForceMeasurements of Platelet Surface with High Spatial Resolution underPhysiological Conditions"Colloid & Interface B, 19, 357-365.

26. Zhu CF, Lee I, Li JW, Wang C, Cao XY, Xu H, Zhang RB (1999) "Atomic Force Microscopy Study ofSmall-size Nanotubular Polymer Thin Films"J. Mat. Res. 14: (3)1084-1090.

25. L.Yang,C.Bai, C.Liu, X.Shi, and I.Lee(1999)"TheoreticalStudies on Conformation Comparison of Braid-like and Triplex DNA"J. Mol. Str., 477, 7-13.

24. 李清，白春礼，Imshik Lee，杨林静，邓文礼，王琛，王乃新，刘次全(1999),溴代胞嘧啶对三螺旋 DNA 稳定性的影响； 计算机与应用化学，16(1), P9-13(In Chinese)

23. 杨林静, 白春礼,李任植,汪新文, 王琛(1998）, Loop 环对分子内三螺旋DNA稳定性的影响; 中国科学B,28(1), P65-70 (InChinese)

22. 杨林静, 李任植, 王琛, 刘峁子, 苏明, 白春礼(1998)，Loop 序列对双分子三螺旋DNA稳定性的影响, 中国科学C, 28(6), P496-500(In Chinese)

21. I.Lee, K-Y.Chan, and D.I.Phillips (1998) "Growthof Electrodeposited Pt Nanocrystals Studied by AFM" Appl. Surf.Sci.,136, 321-330 .

20. I.Lee, K-Y.Chan, and D.I.Phillips (1998) "AFM ofPlatinum Nanoparticles Prepared on HOPG".Ultramicroscopy, 75,69-76.

19. L.Yang, I.Lee, C.Wang, M.Z.Liu, M.Su, and C.Bai (1998) "Influence of Loop Sequenceon Relative Stability of Biomolecular Triplex DNA"Sciencein China C, 41, 381-386.

18. I.Lee, X.Wang, C.Zhu, C.Wang and C.Bai (1998)"AFMImage Analysis of Polystyrene Nanoparticle and DNA-protein Complex with ImageReconstruction"Appl. Surf. Sci., 126, 281-286.

17. L.Yang,X.Fung, I.Lee, C.Bai (1998) "Structure Studies on Host +Guest Recognition Sensory Systems"J Inclus.Phenom. Mol 31, 197-204.

16. L.Yang, I.Lee, C.Wang, Q.Li, C.Bai (1998) "Loop and Water Effect onStability of Intratriplex DNA" J.Mol.Str. 442, 275-280 .

15. L.Yang,X.Fung, I.Lee, C.Bai (1998) "MolecularStructure of Recognition System Host+Guest and DNA Complex" J.Mol.Str.444, 13-20

14. L.Yang,X.Fung, I.Lee, and C.Bai (1997) "StructuralStudies on Host-Guest Recognition Sensory System" Chinese Chem. Lett., 8,707-710.

13. L.Yang,C.Bai,I.Lee, X.Wang, C.Wang(1997) "Effectof Loop on Stability of Intramolecular Triplex DNA" Science in China B. 40,650-656.

12. C.Liu,Z.Zhang, L.Lai, C.Bai, W.Li, R.Wang, I.Lee and Y.Tang (1997)"mRNAProbably Carries The Three-dimensional Genetic Information"ZoologicalResearch (China). 18, 138.

11. I.Lee, W.Deng, C.Wang and C.Bai (1997) "BiphasicTransition of a Hairpin Hexanucleotide Triplex DNA" Biophys.Chem., 67,159-165 .

10. I.Lee, Q.Li, L.Yang, X.Wang, W.Deng, C.Wangand C.Bai (1997) "Polarity Modified Triplex DNA byBromide" Chinese Sci. Bull, 42, 1581-1584.

9. I.Lee, C.Bai and C.Wang (1997) "Scanning TunnellingMicroscopy Studies of Algal Polysaccharide: Gelation of Carrageenan"J.Chin.Electr.Microscopy Soc., 16, 710-713.

8. I.Lee(1997) "X-ray Diffraction Patterns fromOriented Samples of iota- and kappa-carrageenan and Implications forConformational Transition"Polymer (Korea) 21,539-548.

7. I.Lee, C.Bai, C.Wang and X.Wang (1997)  "Computational Analysis of TriplexFormation of Oligonucleotides: Protonated and 5'-methylated Py-Pu-PyMotif" Science in China B, 40(1) 113-121.

6. I.Lee, C.Bai, X.Wang and N.Wang (1997) "Chemical Cross-linkedGelation of HPG under Shear Flow" Polymer J., 29, 17-20.

5. I.Lee, C.Bai and C.Wang (1996) "Conformational Studies ofArabinoglucuronomannoglycan (AGM)" Acta Biophysica Sinica, 12,547-553.

4. C.Wang,C.Bai, X.Li, G.Shang, I.Lee, X.Wang,X.Qiu and F.Tian (1996)"Evidence of DiffusionCharacteristics of Field Emission Electron in Nanostructural Process onGraphite Surface" Appl.Phys.Lett., 69, 348-350.

3. I.Lee (1992) "The Conformational andNano-structural Studies of Macromolecules" PhD Thesis, University ofBristol, UK.

2. I.Lee, E.D.T.Atkins, and M.J.Miles (1992)"Visualisation ofthe Algal Polysaccharide Carrageenan by Scanning Tunnelling Microscopy"Ultramicroscopy, 42/44,1107-1112.

1.M.J.Miles, I.Lee and E.D.T.Atkins (1991) "MolecularResolution of Polysaccharides by Scanning Tunnelling Microscopy"J.Vac.Technol.,B9(2),1206-1209.

Travel Awards for an outstanding young scientists (2001, Congress ofBiomaterials)

Outstanding Paper at 11th annual assembly of Chinese Weed Society,Changsha, China(2013)